Quantitative analysis of bone marrow adipocytes. A. The top panels are images of the distal femurs from 6-month-old and 14-month-old C57BL6/J male mice stained with H&E (magnification ×5). The bottom panels are sections stained with H&E showing the numerous large adipocytes in bone marrow from distal femurs (magnification ×20). B. Area of bone marrow fat infiltration as a % of total area. C. Numbers of bone marrow fat cells (numbers/mm2 bone marrow). Fields were taken from distal femur sections of 6-month-old and 14-month-old mice and calculated using ImagePro software.