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Table 2 Summary of shRNA pool deconvolution by next-generation sequencing

From: A comprehensive platform for highly multiplexed mammalian functional genetic screens

Statistic Even 4x 16x 64x 2-20x
Reads passed filter 22407757 24596943 24793754 24304519 25684516
Mapped reads 22296083 24504006 24687271 24235485 25605983
Unmapped reads 111674 94937 106483 69034 78533
Unmapped reads % 0.5 0.39 0.43 0.28 0.31
1st quartile 40 34 26 35 21
Median 153 149 140 162 107
Mean 315 352.4 351.6 350.7 370.2
3rd quartile 375 408 404 417 385
Max 17960 19360 21750 20000 29460
Uncounted 9784 10748 10045 10983 10739