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Table 1 Summary of the microarray datasets on mastitis infection included in meta-analysis

From: Strengthening insights into host responses to mastitis infection in ruminants by combining heterogeneous microarray data sources

Experiment # (Institution) Host species (# of biological replicates) Pathogen Challenge system Bovine cDNA microarray Time after infection {time point} Signs of infection References
1A (RI/RIBFA) Cattle (4) E. coli Intramammary challenge. Sampled
material: lobulo-alveolar mammary tissue
(in vivo)
ARK-genomics 20 k 6 h {1} No clinical signs and no alteration of TLR2, TLR4,
and β-defensins expressions.
[27, 63, 64]
      12 h {2} Mild clinical signs and small changes of TLR2, TLR4, and β-defensins expressions.  
      24 h {3} Acute clinical signs (including increased SCC count, decreased milk yield, leukopenia, fever, udder swelling) and up-regulation of TLR2, TLR4, and β-defensins expressions  
1B (RI/RIBFA) Cattle (4) S. aureus Intramammary challenge. Sampled material: lobulo-alveolar mammary tissue
(in vivo)
ARK-genomics 20 k 6 h {4} No clinical signs and no alteration of TLR2, TLR4, and β-defensins expressions. [27, 63, 64]
      12 h {5} No clinical signs and no alteration of TLR2, TLR4, and β-defensins expressions.  
      24 h {6} No clinical signs and no alteration of TLR2, TLR4, and β-defensins expressions.  
1C (RI/RIBFA) Cattle (4) S. aureus Intramammary challenge. Sampled material: lobulo-alveolar mammary tissue
(in vivo)
ARK-genomics 20 k 12 h {7} No clinical signs and no alteration of TLR2, TLR4, and β-defensins expressions. [27, 63, 64]
      72 h {8} Acute clinical signs (including increased SCC count, decreased milk yield, leukopenia, fever, udder swelling) and up-regulation of TLR2, TLR4, and β-defensins expressions  
2 (CVI-L) Cattle (3) S. uberis Udder samples containing all layers including epithelia, muscle tissue and mammary gland tissue. In affected samples neutrophils were also present
(in vivo)
ARK-genomics 20 k 36 h-72 h {9} Culling when clear clinical signs were seen. Sample selection from various locations of control and infected mammary gland quarters based on clear microscopic and macroscopic observations -
3 (NSVS) Cattle (6) S. aureus Blood derived primary macrophage cells
(in vitro)
ARK-genomics 17 k 2 h {10} Few genes responding, no cell death. -
      6 h {11} Many genes responding, beginning signs of cell deformation and death  
4 (UNIMI/PTP/CNR) Goat (3) S. aureus Leukocytes in milk
(in vivo)
NBFGC 12 h {12} No clinical signs and no alteration of milk. [65, 66]
      24 h {13} Clear clinical signs (increased SCC count, decreased milk yield, fever)  
5 (INRA) Sheep (8) S. aureus Bone marrow derived primary dendritic cells(in vitro) ARK-genomics 17 k 3 h {14} No cell death. -
      8 h {15} Clear deformation and death of dendritic cells  
6 (UNIMI/PTP/CNR) Goat (10) S. aureus Leukocytes in milk (in vivo) Combi-Matrix 24 h {16} Clinical signs (increased SCC count, decreased milk yield, fever, udder swelling) -
  1. The experimental numbers are reported with the names of the institution where they were conducted, host species and number of replicates, pathogens, challenge systems, microarrays names, time period of observations after infection {in parenthesis the time point #, see also Table 2}, signs of infection, and corresponding references.
  2. Note: ARK-genomics: centre for comparative & functional genomics, Scotland; CNR: Institute of Agricultural Biology and Biotechnology, National Research Council, Italy; CVI-L: Central Veterinary Institute of Wageningen UR, Lelystad, NL; INRA: Institute National de la Recherche Agronomique, France; NBFGC: National Bovine Functional Genomics Consortium, USA; NSVS: Norwegian School of Veterinary Science, Norway; PTP: Parco Tecnologico Padano (PTP), Italy; RI: Roslin Institute and R(D)SVS, University of Edinburgh (UEDIN), UK; RIBFA: Research Institute for the Biology of Farm Animals, Germany; UNIMI: Università degli Studi di Milano, Department of Veterinary Pathology, Hygiene and Public Health, Italy. Microarrays are described in the Materials and Methods section of text.