Table 1 Summary of the microarray datasets on mastitis infection included in meta-analysis
Experiment # (Institution) | Host species (# of biological replicates) | Pathogen | Challenge system | Bovine cDNA microarray | Time after infection {time point} | Signs of infection | References |
|---|---|---|---|---|---|---|---|
1A (RI/RIBFA) | Cattle (4) | E. coli | Intramammary challenge. Sampled material: lobulo-alveolar mammary tissue (in vivo) | ARK-genomics 20 k | 6 h {1} | No clinical signs and no alteration of TLR2, TLR4, and β-defensins expressions. | |
|  |  |  |  |  | 12 h {2} | Mild clinical signs and small changes of TLR2, TLR4, and β-defensins expressions. |  |
|  |  |  |  |  | 24 h {3} | Acute clinical signs (including increased SCC count, decreased milk yield, leukopenia, fever, udder swelling) and up-regulation of TLR2, TLR4, and β-defensins expressions |  |
1B (RI/RIBFA) | Cattle (4) | S. aureus | Intramammary challenge. Sampled material: lobulo-alveolar mammary tissue (in vivo) | ARK-genomics 20 k | 6 h {4} | No clinical signs and no alteration of TLR2, TLR4, and β-defensins expressions. | |
|  |  |  |  |  | 12 h {5} | No clinical signs and no alteration of TLR2, TLR4, and β-defensins expressions. |  |
|  |  |  |  |  | 24 h {6} | No clinical signs and no alteration of TLR2, TLR4, and β-defensins expressions. |  |
1C (RI/RIBFA) | Cattle (4) | S. aureus | Intramammary challenge. Sampled material: lobulo-alveolar mammary tissue (in vivo) | ARK-genomics 20 k | 12 h {7} | No clinical signs and no alteration of TLR2, TLR4, and β-defensins expressions. | |
|  |  |  |  |  | 72 h {8} | Acute clinical signs (including increased SCC count, decreased milk yield, leukopenia, fever, udder swelling) and up-regulation of TLR2, TLR4, and β-defensins expressions |  |
2 (CVI-L) | Cattle (3) | S. uberis | Udder samples containing all layers including epithelia, muscle tissue and mammary gland tissue. In affected samples neutrophils were also present (in vivo) | ARK-genomics 20 k | 36 h-72 h {9} | Culling when clear clinical signs were seen. Sample selection from various locations of control and infected mammary gland quarters based on clear microscopic and macroscopic observations | - |
3 (NSVS) | Cattle (6) | S. aureus | Blood derived primary macrophage cells (in vitro) | ARK-genomics 17 k | 2 h {10} | Few genes responding, no cell death. | - |
| Â | Â | Â | Â | Â | 6 h {11} | Many genes responding, beginning signs of cell deformation and death | Â |
4 (UNIMI/PTP/CNR) | Goat (3) | S. aureus | Leukocytes in milk (in vivo) | NBFGC | 12 h {12} | No clinical signs and no alteration of milk. | |
| Â | Â | Â | Â | Â | 24 h {13} | Clear clinical signs (increased SCC count, decreased milk yield, fever) | Â |
5 (INRA) | Sheep (8) | S. aureus | Bone marrow derived primary dendritic cells(in vitro) | ARK-genomics 17 k | 3 h {14} | No cell death. | - |
| Â | Â | Â | Â | Â | 8 h {15} | Clear deformation and death of dendritic cells | Â |
6 (UNIMI/PTP/CNR) | Goat (10) | S. aureus | Leukocytes in milk (in vivo) | Combi-Matrix | 24 h {16} | Clinical signs (increased SCC count, decreased milk yield, fever, udder swelling) | - |