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Table 1 Properties of the different libraries; abbreviations are as follows: COT cotyledons, E etiolated leaves, L light treated etiolated leaves, EPI epicotyl, LVN.1-5 leaf libraries 1-5, FLO flower, HYP hypocotyl, LVR.1 leaf library, non normalized; asequenced with GS flex, bsequenced with GS 20; all libraries were normalized except LVR.1;

From: Comprehensive transcriptome analysis of the highly complex Pisum sativum genome using next generation sequencing

library

number of raw reads

number of reads after crossmatch clean up

number of reads after MIRA

number reads for the assembly

number of reads with AGI mapping

raw nts

nts after crossmatch

mean readlength raw

mean read length after crossmatch

COTa

343,694

335,050

272,731

272402

220,875

81,050,491

75,679,454

235

225

Ea

144,290

135,906

120,202

120126

101,119

34,849,428

32,229,999

241

237

La

192,117

174,563

157,977

157921

137,359

46,654,732

41,408,162

242

237

EPIa

243,294

233,143

189,093

188193

148,059

54,072,084

50,193,810

222

215

LVN.5a

320,209

311,538

268,315

267966

223,735

77,473,643

72,627,353

241

233

sum/averagea

    

294,100,378

272,138,778

236

228

LVN.1b

350,016

343,361

278,080

277888

228,894

36,551,286

33,767,205

104

98

LVN.2b

302,341

295,416

234,029

233790

192,903

31,399,731

29,009,144

103

98

LVN.3b

146,504

142,862

115,832

115751

96,087

15,049,571

13,963,252

102

97

LVN.4b

278,936

272,367

223,939

223596

184,175

28,590,490

26,562,093

102

97

FLOb

162,353

153,765

95,229

95097

70,957

17,047,889

15,039,288

105

97

HYPb

349,976

327,769

255,437

255310

210,073

36,269,788

31,823,328

103

97

sum/averageb

    

164,908,755

150,164,310

103

97

LVR.1b

308,042

293,201

191,394

191205

167,551

27,779,787

24,062,060

90

82

total

3,141,772

3,018,941

2,402,258

2399245

 

486,788,920

446,365,148

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