Figure 7
Expression of BAHD Acyltransferases in Populus Tissues, Organized by Phylogenetic Relationship. A: Expression of BAHD acyltransferase genes across tissues and genotypes. B: Stress responses of BAHD acyltransferase gene expression across tissues and genotypes. Expression data or ratios (stressed vs. control samples) were log-transformed and visualized in heatmaps (see Methods). Genes are organized by phylogenetic relationship and labelled by the clade color in Figure 1. Genotypes analyzed included: P. fremontii × angustifolia clones 1979, 3200, and RM5, and P. tremuloides clones 271 and L4. Tissues analyzed included: young leaf (YL), expanding leaf (EL), root tips (R), and suspension cell cultures (C). Stress treatments included: nitrogen limitation (low N), leaf wounding (wound, sampled either 1 week or 90 hours after wounding), removal of shoot up to leaf plastochron index three (detop, 90 hours after removal), and methyl jasmonate elicitation (MJ) [24]. White text indicates that raw hybridization intensity for either control (for upregulated genes) or stressed treatment (for downregulated genes) samples was below the quantitation limit (see Methods).