Figure 4

In vivo binding of recombinant AtoC to in silico predicted potential targets of motif 1 in intergenic regions. Results of a ChIP analysis by agarose gel electrophoresis (2% w/v) of PCR amplification products generated by primers corresponding to motif 1 hits and qualified promoters, included in Tables 1 and 5. All ChIP preparations were carried out following acetoacetate induction. Above each PCR product, generated by the specific designated primers, the name of each gene or promoter region comprising the motif sequence is denoted for each pair of lanes. All AtoC additional binding targets are PCR tested (25 cycles) in two ChIP preparations carried out without (-, left lanes) or with (+, right lanes) AcAc induction (AA).