Monitoring the AcAc effect on AtoC in vivo binding to its predicted "atypical" targets. A) All predicted targets matching motifs 2, 3 and 4 (Table 5), that gave a positive signal in genomic DNA, are ChIP analysed. All AtoC additional binding targets are PCR tested (25 cycles) in two ChIP preparations carried out without (-, left lanes) or with (+, right lanes) AcAc induction (AA). Input Chromatin samples are also included representing positive genomic DNA signals for the sampled targets. B) Three selected targets representing each of the afore-mentioned groups (metR-metE, narG and acrD) were also PCR tested together with atoD (25 cycles) using as templates input chromatin controls (Input Chromatin) and immunoprecipitated preparations (ChIPs) from non-induced (-AA) or AcAc induced (+AA) cultures.