Figure 6

Expression of the Hdac1 neurogenic programme is stably attenuated in hdac1 mutant embryos. (A) 12 hpf embryos were sorted according to ascl1b expression level and individuals were then genotyped by PCR. All embryos with strong ascl1b expression had the wild type hdac1 allele (lower band) and were therefore wild-type siblings, whereas embryos with a weak ascl1b expression pattern carried only the mutant hdac1 allele (upper band) and were therefore hdac1 mutants. (B) Similar results to those shown in (A) were obtained for 18 hpf embryos. In both (A) and (B), the forebrain (f) and hindbrain (h) regions of Hdac1-regulated ascl1b expression are indicated. (C) Expression of the Hdac1 neurogenic programme is attenuated in 27 hpf hdac1 mutant embryos. Expression of neurod4, ascl1b, neurod, dlb and lhx9 in 27 hpf hdac1 mutant and wild-type sibling embryos. Expression of each of these genes is reduced in the CNS of hdac1 mutant embryos.