BAC library resources for map-based cloning and physical map construction in barley ( Hordeum vulgare L.)

BMC Genomics

Table 1 New BAC libraries available from cultivar Morex.

BAC-library^1,2	No. of clones	Average insert size (Kb)	Genome coverage	Cloning strategy	Vector	E. coli strain
HVVMRXALLhB³	115,200	93	2.4	Hin dIII, partial	pIndigo BAC5	DH10B
HVVMRXALLhC³	153,600	114	3.4	Hin dIII, partial	pIndigo BAC5	DH10B
HVVMRXALLeA⁴	147,456	126	3.7	Eco RI, partial	pIndigo BAC536	DH10B
HVVMRXALLmA⁵	202,752	143	6	Mbo I, partial	pTAR BAC1.3	DH10B
HVVMRXALLrA⁶	253,440	92	4.7	Mech. Sheared	pSMRT BAC	DH10B

¹Naming follows an agreed nomenclature for Triticeae BAC libraries: three letters species code (H ordeum v ulgare ss. v ulgare), three letters cultivar code (M or ex), three letters genome representation (i.e. ALL = whole genome, 1HS = short arm chromosome 1H), one letter enzyme (i.e. h = Hind III, e = EcoR I, m = Mbo I, r = Random/Mechanically sheared), one letter library identifier in case of multiple libraries per enzyme; ²all libraries are accessible via http://cnrgv.toulouse.inra.fr/en/library/barley; libraries were produced at: ³Australian Centre of Plant Functional Genomics, ⁴Clemson University Genomics Institute, ⁵Childrens Hospital Oakland, ⁶Lucigen corporation.