|
BAC-library1,2
|
No. of clones
|
Average insert size (Kb)
|
Genome coverage
|
Cloning strategy
|
Vector
|
E. coli strain
|
|---|
|
HVVMRXALLhB3
|
115,200
|
93
|
2.4
|
Hin dIII, partial
|
pIndigo BAC5
|
DH10B
|
|
HVVMRXALLhC3
|
153,600
|
114
|
3.4
|
Hin dIII, partial
|
pIndigo BAC5
|
DH10B
|
|
HVVMRXALLeA4
|
147,456
|
126
|
3.7
|
Eco RI, partial
|
pIndigo BAC536
|
DH10B
|
|
HVVMRXALLmA5
|
202,752
|
143
|
6
|
Mbo I, partial
|
pTAR BAC1.3
|
DH10B
|
|
HVVMRXALLrA6
|
253,440
|
92
|
4.7
|
Mech. Sheared
|
pSMRT BAC
|
DH10B
|
- 1Naming follows an agreed nomenclature for Triticeae BAC libraries: three letters species code (H ordeum v ulgare ss. v ulgare), three letters cultivar code (M or ex), three letters genome representation (i.e. ALL = whole genome, 1HS = short arm chromosome 1H), one letter enzyme (i.e. h = Hind III, e = EcoR I, m = Mbo I, r = Random/Mechanically sheared), one letter library identifier in case of multiple libraries per enzyme; 2all libraries are accessible via http://cnrgv.toulouse.inra.fr/en/library/barley; libraries were produced at: 3Australian Centre of Plant Functional Genomics, 4Clemson University Genomics Institute, 5Childrens Hospital Oakland, 6Lucigen corporation.
