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Table 2 Result of insert size determination after Not I-restriction and PFGE analysis.

From: BAC library resources for map-based cloning and physical map construction in barley (Hordeum vulgare L.)

BAC-library

No of clones for NotI restriction

Average insert size (Kb)

No. of clones for HICF

Average fragment number (after HICF)*

HVVMRXALLhA

n.d.

106

10,435

87.9

HVVMRXALLhB

175

93

10,346

96.7

HVVMRXALLhC

181

114

10,279

101.1

HVVMRXALLeA

304

126

10,414

104.1

HVVMRXALLmA

303

143

10,685

123.8

HVVMRXALLrA

366

92

10,679

87.4

  1. In addition the average fragment number after HICF is listed for all BAC libraries based on a random set of investigated clones. * Size standard = GS1200LIZ (Applied Biosystems); n.d. = not determined