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Table 2 Result of insert size determination after Not I-restriction and PFGE analysis.

From: BAC library resources for map-based cloning and physical map construction in barley (Hordeum vulgare L.)

BAC-library No of clones for NotI restriction Average insert size (Kb) No. of clones for HICF Average fragment number (after HICF)*
HVVMRXALLhA n.d. 106 10,435 87.9
HVVMRXALLhB 175 93 10,346 96.7
HVVMRXALLhC 181 114 10,279 101.1
HVVMRXALLeA 304 126 10,414 104.1
HVVMRXALLmA 303 143 10,685 123.8
HVVMRXALLrA 366 92 10,679 87.4
  1. In addition the average fragment number after HICF is listed for all BAC libraries based on a random set of investigated clones. * Size standard = GS1200LIZ (Applied Biosystems); n.d. = not determined