The enrichment of HCNEs around conserved human ST miRNAs (including ST miRNAs overlapping with GRBs). Figure 2 shows the cumulative curves of HCNE density in five lineage comparisons. The lineages compared are indicated at the top of each figure. The HCNE density was calculated based on a 300 kb window centered on a region of interest, which is either a ST miRNA, a randomly selected coding/non-coding region (control sets) or a GRB target gene. The x-axis shows the percentage of base pairs in HCNEs within the 300 kb window (HCNE density). The fraction of 300 kb windows we analyzed is shown in the y-axis. The red curve shows the HCNE density of the conserved human ST miRNAs, while the grey, blue and green curves show the HCNE density of the non-coding and protein-coding control sets as well as the set of GRB target genes, respectively. Conserved human ST miRNAs are therefore more likely to be located in regions with higher HCNE density than would be expected by chance.