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Table 3 Verification of the microarray data by qRT-PCR

From: Global analysis of gene expression in response to L-Cysteine deprivation in the anaerobic protozoan parasite Entamoeba histolytica

Common Name

Accession Number

Fold Change

  

3 h

6 h

12 h

24 h

48 h

Major fascilitator superfamily (MFS) transporter

XM_647419

2.0

12.8

7.2

4.2

2.3

  

(4.1)

(14.6)

(9.9)

(4.7)

(2.6)

Iron sulfur flavoprotein (ISF)

XM_650038

1.8

4.4

14.9

5.5

2.8

  

(3.6)

(6.8)

(9.8)

(5.4)

(4.2)

NADPH-dependent oxidoreductase (EhNO2)

XM_648481

-3.0

-3.0

-6.0

-8.4

-7.8

  

(-1.9)

(-1.9)

(-3.0)

(-10.6)

(-8.3)

Hypothetical protein

XM_645369

-2.2

-6.4

-12.9

-3.4

-2.7

  

(-1.1)

(-6.3)

(-11.1)

(-3.0)

(-2.6)

RNA polymerase II 15-kDa subunit

XM_643999

1.0

-1.2

1.1

-1.3

-1.3

  

(1.4)

(1.2)

(1.2)

(1.1)

(1.2)

  1. The common names, accession numbers, and fold changes of the selected genes upon L-cysteine deprivation are shown. The upper values are the fold changes in the expression obtained from qRT-PCR. The corresponding fold changes in the expression values obtained from Affymetrix analysis are shown in brackets.