Skip to main content

Table 4 Primers used to amplify 10 candidate genes by qRT-PCR

From: Identification of genes differentially expressed in a resistant reaction to Mycosphaerella pinodes in pea using microarray technology

Gene

Forward primer (5'-3')

Reverse primer (5'-3')

Reference accession

Peroxidase (PsOXII)

cttggaggacccacatggat

tttggcttgctgttcttgca

GenBank:AB193816.1

Disease resistance response protein 39 (DRR230-b)

gggagtatgcttcacgaatgc

ttttgagtgcagaaacatttcca

GenBank: LO1579.1

12-oxophytodienoic acid 10,10-reductase (OPR1)

aagtgaatgacagaaccgatga

atggaaaccgacagcgatt

GenBank: AY954368.1

Glutathione S-transferase

gttcgtcctcctccgctaact

gttcgtcctcctccgctaact

GenBank: AB087837

Nine-cis-epoxycarotenoid dioxygenase 4 (nced4)

ctctcttcccgaacgtcttctc

cgcacgtggatccataaccgcc

GenBank: U69554.1

6a-hydroxymaackiain methyltransferase (hmm6)

tttgaactttgttggtggagatatg

aatcatgcagaacccacttgagt

GenBank: U69554.1

Ferrodoxin NADP oxidoreductase

acaagcaagtgtgccgaaagt

ggaggttcagaaaggattttcca

GenBank: X99419.1

Chlorophyll a/b-binding protein

gttttcgcatcaacggactt

attgcccaccagggtaaag

GenBank: EF488077.1

GA protein

tgcagacagctttaacctttgc

tgcgagacactctttggtgttg

GenBank: X65154.1

Ribulose 1 5-bisphosphate carboxylase small subunit

caagtcttgaaggagcttgatgaa

gttgtcgaaaccgatgatacga

GenBank: J01257.1