Figure 2

Domain shuffling has acted to form rhesus macaque KIR genes. The results of a domain-by-domain phylogenetic analysis are shown schematically. Predicted amino-acid consensus sequences for each of the genes were used to form both neighbor-joining and parsimony trees as described in the Methods section. The sequences encoding the three extracellular Ig-like domains were analyzed separately. Boxes are colored for the D0, D1, and D2 domains where the grouping was supported by >50% bootstrap support in the phylogenetic analyses. Boxes that are not completely colored represent cases where support of >50% was only found in the neighbor-joining analysis. Long cytoplasmic tails are colored red and short tails are colored green. The domains colored white were not resolved into any group in the analysis and should not be interpreted as being closely related to each other. The stem, transmembrane and cytoplasmic tails were grouped as either long or short by inspection of the alignment. D0, D1, and D2 denote the Ig-like domains. ST/TM/CYT denotes the stem, transmembrane, and cytoplasmic tail domains.