De novo sequence assembly and characterization of the floral transcriptome in cross- and self-fertilizing plants

Table 2 Summary statistics for reference transcriptome through progressive stages of assembly.

Assembly stage	*N50*	Mean contig size	Total length	Number of contigs
Oases assembly	611	374.1	21,311,238	56,971
Reciprocal BLAST	807	703.5	15,919,812	22,630
Contig elongation	812	709.9	16,065,194	22,630
Reduced fragmentation	819	717.8	15,995,086	22,282
Final (including singletons)¹	1,129	884.3	23,869,762	26,994

¹ Singletons over 1000 bp with BLASTx hits where e < 1 × 10^-15 are included in the final consensus
For the first stage all statistics are the average across the four independently sequenced genotypes.

ISSN: 1471-2164