Figure 9

Confirmation of the CGI tiling array using bisulfite sequencing PCR. A. Methylation level at each CpG site was measured in 3 independent tumors and 3 independent control samples for coro2ba ± SEM (data for angptl1 and igfbp1b is not shown; T = tumor, C = control, region: -580 to -879). B. Percentage of DNA methylation (combined for all measured CpGs) in angptl1, igfbp1 and coro2ba genes showed significant changes between tumor and control samples (p-value < 0.01). (angptl1: tumors: number of CpG sites = 21, controls: number of CpG sites = 21; igfbp1b:tumors: number of CpG sites = 15, controls: number of CpG sites = 15; coro2ba: tumors: number of CpG sites = 41, controls: number of CpG sites = 41 ± SEM).