Table 1 Identified genes with altered methylation in zebrafish HCC.

A. Hypomethylated Genes

   Proliferation

   Kruppel-like factor 12b

   Insulin-like growth factor binding protein 5a

   Insulin-like growth factor binding protein 2a

   Insulin-like growth factor binding protein 1b

   Insulin-like growth factor-binding protein 2A precursor

   Estrogen related receptor delta fragment

   Hypothetical protein LOC550398

   Stress

   Similar to SH2 domain containing 3C

   PI-kinase-related SMG-1

   Glycolysis

   Enolase 2

   Hexokinase 1

   Cell cycle, metastasis, adhesion, cell growth, stress

   c-Jun protein

   BCL2-associated athanogene 5

   Angiopoietin-like 3

   Angiopoietin-1 receptor precursor

   Ras homolog gene family, member Ua

   Menage a trois homolog 1

   Serine/threonine and tyrosine protein kinase

   DNA binding and regulation of transcription

   DNA (cytosine-5-)-methyltransferase 6

   Leucine zipper protein 2 precursor

   Histone H2A

   Histone deacetylase 4

   Homeobox protein Hox-B5a

   Pancreas transcription factor 1 subunit alpha

   Hypothetical protein LOC692291

   Metastasis associated 1 family, member 2

   Homeobox protein Hox-B4a

   Lysine-specific demethylase 4A

   RAB11 family interacting protein 4 (class II) a

B. Hypermethylated Genes

   Anti-angiogenesis

   Hematopoietically-expressed homeobox protein hhex

   Cell-cell adhesion

   Hypothetical protein LOC678612

   Novel protocadherin protein fragment

   Transporter

   ATP-binding cassette, sub-family A, member 5

   immune system

   Novel protein similar to nuclear factor, interleukin 3 regulated

   Novel protein fragment

   C5a anaphylatoxin chemotactic receptor

   Angiogenesis and oxidative stress protection

   Vascular endothelial zinc finger 1

   Membrane

   Coronin, actin binding protein 2ba