Figure 1
From: A tissue-specific landscape of sense/antisense transcription in the mouse intestine
Transcript length dependent coverage bias along 5'-3' transcript axis. Transcript length-normalized coverage per base was plotted for several transcript classes. The graph depicts the small intestine data set for oligodT-primed cDNA (SMART protocol) starting from (A) oligodT-purified mRNA and (B) total RNA. Note that especially transcripts > 5000 bp display a stronger bias due to relative overrepresentation of the 3'end.