Strategy for detection and verification of polyadenylated nTARs. (A) All obtained reads from the screening protocol were matched against the mouse genome followed by generation of coverage tracks (I). Covered regions, which are not consistent with either current RefSeqGene or ensGene gene annotation were identified and selected (II). Only nTARs fulfilling our quality criteria (length ≥ 50 bp, average base coverage ≥ 3, III) were further processed and had to be confirmed by a second RNA-Seq protocol with at least 3 reads (IV). (B) An example of a nTAR is shown: extension of the last exon of Nek7 (NIMA (never in mitosis gene a)-related kinase 7), here shown for small intestine (orientation of reads shown by colour, modified screenshot from ucsc genome browser).