Figure 4

Gene phylogeny and amino acid alignment of PGRP protein sequences. (A) A bayesian phylogenetic tree of insect PGRP proteins. Bayesian posterior probabilities are shown for all major nodes supported with probability higher than 60%. (B) Amino acid alignment of the 6 predicted proteins from Galleria together with predicted protein sequences deduced from publicly available insect sequence datasets. Amino acid sequence alignments were performed using MAFFT multiple alignment program without the predicted signal peptide and part of the N-terminus as in some cases only partial sequence information was available. Identical residues are boxed with dark shading, and conserved residues are boxed with light shading. Species abbreviations: Manduca sexta (Mse), Bombyx mori (Bmo), Trichoplusia ni (Tni), Phlebotomus papatasi (Ppa), Samia cynthia ricini (Scr), Tribolium castaneum (Tca), Glossina morsitans (Gmm), Galleria mellonella (Gme), Tenebrio molitor (Tmo).