Figure 3

Reverse-transcriptase PCR validation of gene-specific cDNA expression in larval and alevin stages of development. Development stage indicated by day (Days 38 through 77) post-fertilization. For determining the lengths of each amplicon, the strongest marker bands indicate fragment lengths of 500 bp (cyp19a, cyp19b1, foxl2b, mis and sf1) or 1000 bp (dax1, dax2 and foxl2a).