Figure 6

Real-time PCR analysis of genes detected in VSa13 (A) and VSa16 (B) cells selected for validation. Genes were selected according to the following criteria: 2 genes with 1.5-2 FC, 2 genes with 2-10 FC, and 2 genes with FC higher than 10. All changes in gene expression evaluated by qPCR were significant according to Student's test (p < 0.05) unless otherwise stated (non-significant - #). Genes selected for analysis were: i) secreted phosphoprotein 1 (SPP1; osteopontin), photoreceptor outer segment all-trans retinol dehydrogenase (short-chain dehydrogenase reductase; SDR), BMP-2, tissue non-specific alkaline phosphatase (TNAP), cell division protein kinase 10 (CDPK-10), S100 (calcium-binding), periostin precursor (PER), retinoic acid receptor β (RAR-β), suppressor of cytokine signaling 3 (SOCS-3), MGP, plasma retinol-binding protein precursor (PRBP) and betaine homocysteine methyltransferase (BHMT) in VSa13 cells; ii) SPP1, SDR, Ras GTPase-activating-like protein (RGAP), TNAP, stathmin 1/oncoprotein 18 (STMN-1), S100, SOCS-3, Ras-related protein 1A (Rap-1A), PER, MGP, PRBP and BHMT in VSa16 cells.