Methylation of distal intergenic regions. A, Methylated and hypermethylated distal intergenic regions were highly enriched for overlap with sequences with a high degree of conservation in mammalian and vertebrate species, as indicated by phastCons scores > 0.8. B, Conserved transcription factor binding sites are highly enriched in the methylated and hypermethylated regions. A - B, Conventions are the same as for Figure 2A. C, DNA methylation signals surrounding a hypermethylated region that was not near any known genes in the hg15 genome build. In addition to the annotations described in Figure 2B, the phastCons scores, representing the degree of conservation across 28 vertebrate and mammalian species, is shown. D, Bisulfite sequencing verification of the boxed region from (C), following the same conventions at Figure 2C. Due to space limitations, only the summary schematics are shown. E, Normalized expression of psiTPTE22, a newly annotated pseudogene in hg18 that arises just downstream of the hypermethylated region shown in Figure 5C-D, in PrEC, DMSO (Ctl) and 1 μM AZAdC treated LNCaP cells.