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Figure 3 | BMC Genomics

Figure 3

From: Turbot reovirus (SMReV) genome encoding a FAST protein with a non-AUG start site

Figure 3

FAST proteins encoded by SMReV and GCRV. (A) Genome segment S7 of SMReV encodes a FAST protein. CIK cells were transfected with plasmids expressing the full length, 5' fragment (1-613), or NS32 of S7, respectively. Expression of full length or 5' fragment (1-613) of S7 induced syncycium formation (panel a 1-2 and panel c 1-2), but no syncycium formation was observed in cells expressing NS32 (panel b 1-2). (B) Determining the translation start site of NS22. The 5' terminal sequence of S7 is shown at top of the figure. The 5' fragment of S7 is schematically indicated by a horizontal line comprising bases 1-613 (positions numbered on the top). A similar horizontal bar indicates each truncation or mutation. The ability of each construct to form a syncytium is indicated as positive (+) or negative (-). Representative images of transfected CIK cells are presented at the right side of the figure. (C) Cell-cell fusion induced by NS16 encoded by S7 of GCRV. CIK cells were transfected with plasmid pcDNA3.1-NS16 and pEGFP-NS16 respectively.

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