Figure 1

Histochemical analysis of C. truncatum infected lentil leaflet tissues. (A) Plasmolyzed (0.8 M NaNO₃) infected cells of lentil cultivar 'Eston' stained with Neutral Red (0.1% in water) vital dye show that the intracellular primary hyphae of C. truncatum do not form any plant-pathogen interface and remain separated during the biotrophic phase. C, conidia; A (*), appressorium; IV, infection vesicle; PH, primary hyphae; and PM, plasma membrane. (B) Infected leaflet cells of Eston display the transition from the biotrophic phase to necrotrophic phase of C. truncatum. BNS, biotrophy-necrotrophy switch; and SH, secondary hyphae. Bars = 10 μm.