EST mining identifies proteins putatively secreted by the anthracnose pathogen Colletotrichum truncatum

Bhadauria, Vijai; Banniza, Sabine; Vandenberg, Albert; Selvaraj, Gopalan; Wei, Yangdou

doi:10.1186/1471-2164-12-327

BMC Genomics

Table 1 C. truncatum unigenes encoding secretory proteins

From: EST mining identifies proteins putatively secreted by the anthracnose pathogen Colletotrichum truncatum

Unique sequence ID	Accession	Putative function	Organism	E value
*Cell envelop associated protein*
Contig 1	XP_002144203	GPI anchored serine-threonine rich protein	Penicillium marneffei	9e-11
Contig 2	XP_001269791	GPI anchored serine-threonine rich protein	Aspergillus clavatus	1e-06
Contig 3	XP_002148880	GPI anchored protein, putative	Penicillium marneffei	1e-14
Contig 4	XP_002850839	GPI anchored serine-rich protein	Microsporum canis	5e-11
Ct21-4350	XP_002144203	GPI anchored serine-threonine rich protein	Aspergillus fumigatus	8e-11
Ct21-949	EEY14502	GPI-anchored cell wall organization protein Ecm33	Verticillium albo-atrum	4e-49
Ct21-1020	EEY23888	GPI transamidase component Gpi16	Verticillium albo-atrum	4e-100
Ct21-3268	XP_750946	CFEM domain-containing protein	Glomerella graminicola	4e-40
Contig 7	CAQ16271	Hypothetical protein	Glomerella graminicola	1e-20
Ct21-4487	CAQ16270	Hypothetical protein	Glomerella graminicola	7e-18
Contig 9	XP_001557501	Predicted protein	Botryotinia fuckeliana	9e-09
Ct21-2424	CAA04765	Glycoprotein CIH1	Glomerella lindemuthiana	2e-27
Ct21-2435	CAA04765	Glycoprotein CIH1	Glomerella lindemuthiana	2e-22
Ct21-3485	CAA04765	Glycoprotein CIH1	Glomerella lindemuthiana	8e-29
Ct21-1783	XP_960686	Clock-controlled protein 6	Neurospora crassa	1e-12
Ct21-4525	XP_960686	Clock-controlled protein 6	Neurospora crassa	1e-11
Contig 29	XP_960686	Clock-controlled protein 6	Neurospora crassa	3e-11
Ct21-3233	XP_002340703	Extracellular conserved serine-rich protein	Talaromyces stipitatus	1e-19
Contig 11	XP_746687	Conserved glycine-rich protein	Aspergillus fumigatus	3e-22
Ct21-160	XP_775248	Hypothetical protein	Glomerella graminicola	9e-08
Ct21-3817	XP_365520	Hypothetical protein	Magnaporthe grisea	1e-50
Ct21-3527	XP_001910524	Hypothetical protein	Podospora anserina	2e-05
Contig 28	XP_001264578	MFS sugar transporter	Neosartorya fischeri	6e-87
Contig 30	XP_389647	Glucose-regulated protein homolog precursor GRP 78	Gibberella zeae	0.0*
Ct21-2300	AAM00019	ToxB precursor	Pyrenophora tritici-repentis	3e-04
Ct21-3042	EEY18399	Cu-Zn superoxide dismutase	Verticillium albo-atrum	1e-45
Ct21-3890	EEY18083	Peptidyl-prolyl cis-trans isomerase B	Verticillium albo-atrum	3e-81
Ct21-4099	EEY20068	Chitin deacetylase	Verticillium albo-atrum	8e-83
Ct21-4275	XP_001831864	Metalloreductase	Coprinopsis cinerea	4e-14
Ct21-1979	EEY19380	Integral membrane protein	Verticillium albo-atrum	3e-43
Contig 12	XP_001553407	Predicted protein	Botryotinia fuckeliana	0.015*
Ct21-43	EEY14873	Conserved hypothetical protein	Verticillium albo-atrum	6e-69
Ct21-2181	XP_001906422	Hypothetical protein	Podospora anserina	1e-23
Ct21-1105	XP_387661	Hypothetical protein	Gibberella zeae	9e-39
Ct21-2709	XP_387661	Hypothetical protein	Gibberella zeae	1e-35
Ct21-3808	XP_364081	Hypothetical protein	Magnaporthe grisea	9e-21
Ct21-4329	XP_001592593	Hypothetical protein	Sclerotinia sclerotiorum	5e-18
*Candidate effectors*
Contig 6	EEY19659	Conserved hypothetical protein	Verticillium albo-atrum	7e-29
Contig 8	ABE73692	Eliciting plant response-like protein	Hypocrea atroviridis	2e-45
Contig 10	XP_386615	Hypothetical protein	Gibberella zeae	2e-80
Contig 31	EEU39436	Small secreted protein	Nectria haematococca	1e-07
Ct21-741	EEY14856	Conserved hypothetical protein	Verticillium albo-atrum	9e-36
Ct21-1631	EEY16152	Conserved hypothetical protein	Verticillium albo-atrum	5e-59
Contig 32	ABK76310	Hypersensitive response-inducing protein	Ceratocystis ulmi	7e-23
Ct21-2867	EEU38174	Hypothetical protein	Nectria haematococca	4e-36
Ct21-4630	EEU46355	Predicted protein	Nectria haematococca	1e-45
Contig 5	ACF19427	Extracellular protein 6	Passalora fulva	0.028*
Ct21-1573	ACF19427	Extracellular protein 6	Passalora fulva	1e-22
*Hydrolases*
Contig 13	EEY15172	Aminopeptidase Y precursor, putative	Verticillium albo-atrum	5e-75
Contig 14	XP_956796	Carboxypeptidase Y precursor	Neurospora crassa	9e-108
Contig 15	ACM42424	Subtilisin serine protease	Chaetomium globosum	1e-61
Ct21-125	B8XGR2	Carboxypeptidase 2	Trichophyton equinum	4e-19
Ct21-572	EEY23108	Serin endopeptidase	Verticillium albo-atrum	1e-42
Ct21-708	AAS45251	Subtilisin-like serine protease	Verticillium dahliae	4e-27
Ct21-1114	XP_002843648	Glutamate carboxypeptidase 2	Arthroderma otae	5e-110
Ct21-1766	EEY22919	Peptidase M14	Verticillium albo-atrum	4e-99
Ct21-1999	XP_001940792	Carboxypeptidase Y precursor	Pyrenophora tritici-repentis	5e-44
Ct21-2223	EEY15034	Metalloprotease	Verticillium albo-atrum	1e-49
Ct21-2830	ACV96842	Aspartic proteinase	Botryotinia fuckeliana	4e-63
Ct21-3428	CAL25580	Serin endopeptidase	Hypocrea lixii	2e-72
Ct21-3969	CAB44651	Chymotrypsin	Metarhizium anisopliae	3e-62
Ct21-4220	EEY23006	Conserved hypothetical protein	Verticillium albo-atrum	2e-88
Ct21-4840	EEY23418	Serin endopeptidase	Verticillium albo-atrum	2e-22
Ct21-4964	EEY21352	Aminopeptidase Y	Verticillium albo-atrum	1e-52
Contig 16	EDP48344	Pectin lyase B	Aspergillus fumigatus	5e-75
Contig 17	XP_001935104	Cutinase precursor	Pyrenophora tritici-repentis	6e-60
Contig 18	O94218	Xyloglucan-specific endo-beta-1,4-glucanase precursor	Aspergillus aculeatus	5e-37
Contig 19	ABC65824	Cell wall glucanosyltransferase	Metarhizium anisopliae	3e-65
Contig 20	EEY20211	Cutinase-2	Verticillium albo-atrum	3e-38
Contig 21	XP_002382421	Esterase, putative	Aspergillus flavus	2e-26
Ct21-1152	XP_002373471	Lipase/esterase, putative	Aspergillus flavus	9e-25
Ct21-1372	XP_002379112	Esterase, putative	Aspergillus flavus	6e-46
Ct21-2524	EEY22945	Hydrolase (Esterase/Lipase domain)	Verticillium albo-atrum	3e-27
Ct21-2039	EEY15257	Pectinesterase	Verticillium albo-atrum	1e-78
Contig 25	CAC29255	Pectin methyl esterase	Botryotinia fuckeliana	1e-14
Contig 22	EEY23761	Pectate lyase	Pyrenophora tritici-repentis	6e-79
Contig 23	XP_001933274	Endoglucanase II	Pyrenophora tritici-repentis	5e-61
Contig 24	XP_001940096	Glycosyl hydrolase	Pyrenophora tritici-repentis	5e-42
Contig 26	EEU40344	Glycoside hydrolase family 43	Nectria haematococca	2e-117
Contig 27	XP_001261592	Pectate lyase, putative	Neosartorya fischeri	3e-39
Ct21-435	EEY22330	Cell wall glycosyl hydrolase	Verticillium albo-atrum	2e-137
Ct21-551	XP_001940634	Periplasmic beta-glucosidase precursor	Pyrenophora tritici-repentis	2e-98
Ct21-605	EEY19750	Septation protein SUN4	Verticillium albo-atrum	3e-42
Ct21-657	EEU42734	Glycoside hydrolase family 76	Nectria haematococca	7e-46
Ct21-658	XP_001939866	Arabinosidase	Pyrenophora tritici-repentis	3e-177
Ct21-817	EEY20388	Cell wall glycosyl hydrolase	Verticillium albo-atrum	2e-120
Ct21-1102	NP_592836	Cell wall protein Asl1, O-glucosyl hydrolase	Schizosaccharomyces pombe	1e-12
Ct21-1170	CAC14022	Endopolygalacturonase	Colletotrichum gloeosporioides f. sp. malvae	1e-78
Ct21-1507	ABC65824	Cell wall glucanosyltransferase	Metarhizium anisopliae	8e-56
Ct21-1571	EEY17994	Beta-glucanase	Verticillium albo-atrum	3e-109
Ct21-1584	AAQ23181	Extracellular lipase	Gibberella zeae	5e-66
Ct21-1644	EEY23761	Pectate lyase	Verticillium albo-atrum	3e-50
Ct21-1737	EEY19876	Beta-glucosidase	Verticillium albo-atrum	1e-3
Ct21-1738	EEY23761	Pectate lyase	Verticillium albo-atrum	3e-50
Ct21-1739	XP_001937817	Fungal cellulose binding domain containing protein	Pyrenophora tritici-repentis	3e-34
Ct21-3571	XP_001937817	Fungal cellulose binding domain containing protein	Pyrenophora tritici-repentis	9e-35
Ct21-4548	XP_001937817	Fungal cellulose binding domain containing protein	Pyrenophora tritici-repentis	3e-40
Ct21-1786	EEY20352	Phosphoserine phosphatase	Verticillium albo-atrum	2e-112
Ct21-1816	BAH10648	Beta-L-arabinopyranosidase/alfa-D-galactopyranosidase	Fusarium oxysporum	2e-81
Ct21-1965	EEU44929	Glycoside hydrolase family 12	Nectria haematococca	4e-40
Ct21-1989	ACZ06599	Exopolygalacturonase	Fusarium oxysporum f. sp. lycopersici	1e-96
Ct21-2762	XP_001216041	Endoglucanase I precursor	Aspergillus terreus	7e-09
Ct21-2876	XP_958254	Endoglucanase IV precursor	Neurospora crassa	5e-56
Ct21-3095	EEY21635	Mannan endo-1,4-beta-mannosidase	Verticillium albo-atrum	2e-103
Ct21-3342	EEY20111	Glycosyl hydrolase family 43 protein	Verticillium albo-atrum	5e-119
Ct21-3508	AAM77705	Endoglucanase	Nectria ipomoeae	6e-66
Ct21-3569	ABH03046	Pectin lyase I	Penicillium occitanis	4e-63
Ct21-3767	XP_001259883	Extracellular cellulase CelA, putative	Neosartorya fischeri	3e-54
Ct21-3948	EEY21541	Alpha-galactosidase	Verticillium albo-atrum	1e-115
Ct21-4357	XP_001937210	Beta-1,6-galactanase	Pyrenophora tritici-repentis	1e-17
Ct21-4443	XP_001836535	Endoglucanase B	Coprinopsis cinerea	6e-43
*Other proteins*
Ct21-153	AAL08969	Glycine-rich protein	Coccidioides posadasii	1e-27
Ct21-1373	EFQ36857	NUDIX-domain containing protein	Glomerella graminicola	1e-46
Ct21-1408	CAD71220	Chloroperoxidase related protein	Neurospora crassa	1e-46
Ct21-3930	EEH44533	Glutaminase	Paracoccidioides brasiliensis	6e-62
Ct21-4401	EEY18112	L-ascorbate peroxidase	Verticillium albo-atrum	4e-06
Ct21-4758	EEY17786	Predicted protein (NAD(P)(+)-binding protein region)	Verticillium albo-atrum	0.006*
Ct21-3336	XP_360331	Predicted protein	Magnaporthe grisea	6e-05
Ct21-25	EEY16153	Conserved hypothetical protein	Verticillium albo-atrum	3e-60
Ct21-695	EEU39144	Hypothetical protein	Nectria haematococca	2e-21
Ct21-1015	EEU42792	Hypothetical protein	Nectria haematococca	4e-23
Ct21-1283	CAQ16193	Hypothetical protein	Glomerella graminicola	2e-05

* Unigenes with E value > 10^-5 are classified according to their conserved signatures as described in the text.

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ISSN: 1471-2164

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