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Figure 7 | BMC Genomics

Figure 7

From: Genome-wide expression quantitative trait loci (eQTL) analysis in maize

Figure 7

Unexpected expression regulation of a pseudogene results from sequence variation in the carboxy terminal of glutamine amidotransferase. A The B73 and Mo17derived sequences for the trans-regulator glutamine amidotransferase code for nearly identical proteins. Blue boxes highlight residues different from the B73 reference sequence. Fine mapping of the eQTL to a 186 bp interval determined that the carboxy-terminus accounts for the trans-regulation, black bar. Class I glutamine amidotransferase proteins require the conserved C-H-E triad (red boxes) for their expected enzymatic functions suggesting that, despite the sequence differences between the genotypes, the proteins likely remain functional in their expected pathway in situ. B Genomic structure on chromosome 1(BAC AC177817) that encodes for the pseudogene is derived from chimerization of 1.5 exons of the functional ABA 8'-hydroxylase genic region (blue bar, blue exons) of chromosome 4 (BAC AC182187), a 5' element enabling transcription originating from chromosome 10 (green bar; BAC AC194847), and genomically non-unique sequence (yellow dashed bar, yellow exons). The microarray probe (red bar) used to measure the pseudogene is unique within the transcriptome.

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