Figure 3

Temporal characterisation of the prostaglandin response. Time course response to macrophages stimulated during 0, 30 min, 1, 3, 6, and 12 h with 10 μg/mL of PGN O111:B4 and K12. Experiments were performed in independent groups of PGN-stimulated (n = 3) or control macrophage cultures (n = 9). a) COX-2 and PTGDS mRNA abundance over time in response to PGN-B4 (black bar) or PGN-K12 (white bar). Were observed significative differences in the mRNA abundance between the times and treatments (PGNs) in both genes (two way ANOVA p < 0.01). b) PGE₂ and PGD₂ release (pg/mL) stimulated by PGN-O111:B4 (black bars), PGN-K12 (white bars) and control (grey bars) into the culture medium (n = 3/treatment). Were observed significative differences in the release between the times and treatments (PGNs or control) by both prostaglandin (two way ANOVAs p < 0,01). The results are presents as fold change relative to 18S abundance and ± std deviation.