Figure 4

Concentration dependence of the prostaglandin response. Dose response (0.1, 1, and 10 μg/mL) of trout macrophages to PGN O111:B4 and K12 challenge. Experiments were performed overnight in independent macrophage cultures (n = 3). a) COX-2 and PTGDS mRNA abundance (black bar) in response to different doses of PGN-O111:B4 or PGN-K12 (0.1, 1, 10 μg/mL). Were observed significative differences in the mRNA abundance between different doses and treatments (PGNs) in both genes (two way ANOVAs p < 0.01). b) PGE₂ and PGD₂ release (pg/mL) into the culture medium (grey bars). Were observed significative differences in the release between doses and treatments (PGNs or control) by both prostaglandins (two way ANOVAs p < 0.01). The results are presented as fold change relative to 18S abundance and mean ± std deviation.