Divergent responses to peptidoglycans derived from different E. coli serotypes influence inflammatory outcome in trout, Oncorhynchus mykiss, macrophages

Table 1 Summary of selected transcripts expressed after challenges with PGN-O111:B4

	Early		Median		Late
Antigen presenttion	Mean	SD	Mean	SD	Mean	SD
MHC class I heavy chain-1	4.92	2.35	3.27	1.67	n/s	n/s
Macrophage receptor MARCO	2.07	0.56	5.02	1.75	n/s	n/s
Cysteine-rich protein 1	-3.59	0.74	n/s	n/s	n/s	n/s
BPI binding protein	n/s	n/s	11.93	5.78	n/s	n/s
Cell adhesion and proliferation
CD166	1.79	0.35	4.99	2.97	3.06	1.48
Cytokines and Chemokines
C-C chemokine receptor type 3	4.25	3.47	n/s	n/s	n/s	n/s
Chemokine receptor CXCR4	n/s	n/s	-4.24	1.01	n/s	n/s
Cellullar defense response
N-acetylmuramoyl-L-alanine amidase	-1.60	0.05	1.46	0.03	1.46	0.17
Peroxiredoxin 1-1	-2.98	1.42	1.30	0.76	1.74	0.30
Interleukin enhancer-binding factor 3	n/s	n/s	2.39	1.47	n/s	n/s
TNF decoy receptor	n/s	n/s	11.42	3.86	12.09	10.98
NF-kappaB inhibitor alpha-3	n/s	n/s	9.24	6.05	n/s	n/s
Myeloid differentiation primary response	n/s	n/s	n/s	n/s	1.56	0.29
Phosphotyrosine SH2 domain	n/s	n/s	n/s	n/s	2.86	1.17
Procathepsin L-1	n/s	n/s	4.11	1.67	n/s	n/s
Procathepsin L-2	n/s	n/s	3.47	1.28	n/s	n/s
Cathepsin B-2	n/s	n/s	3.36	2.29	n/s	n/s
Cathepsin D-2	n/s	n/s	3.99	0.25	n/s	n/s
Cathepsin C-1	n/s	n/s	3.28	1.16	n/s	n/s
Cathepsin C-2	n/s	n/s	5.14	5.30	n/s	n/s
MAPK/ERK
Serine/threonine-protein kinase 2	n/s	n/s	5.03	2.65	n/s	n/s
MAPK/ERK kinase kinase 5-1	n/s	n/s	-1.68	0.33	n/s	n/s
C-Jun protein	n/s	n/s	n/s	n/s	3.99	1.62
MAPK/ERK kinase kinase 1-2	n/s	n/s	n/s	n/s	1.87	0.46
MAPK kinase 9-2	n/s	n/s	n/s	n/s	5.78	3.73

Transcripts represented were firstly selected for expression level (p < 0.01) and then implication in biological processes related to PGN stimulation (immune/inflammatory responses) during PGN-O111:B4. n/s: not signal, Mean: Fold expression average (n = 3), SD: standard deviation.

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