Table 1 Alfalfa sequence length distribution pre-and post-assembly
Pre-assembly | Chilean | Wisfal | Combined |
|---|---|---|---|
Total base count (bp) | 57,563,286 | 67,698,210 | 125,261,496 |
Sequence count | 155,120 | 177,871 | 332,991 |
100-200 bp | 13,881 | 10,380 | 24,261 |
200-300 bp | 23,360 | 22,388 | 45,748 |
300-400 bp | 40,842 | 55,262 | 96,104 |
400-500 bp | 65,222 | 79,475 | 144,697 |
500-600 bp | 11,806 | 10,362 | 22,168 |
600-700 bp | 9 | 4 | 13 |
Minimum length (bp) | 100 | 100 | 100 |
Maximum length (bp) | 621 | 636 | 636 |
Average length (bp) | 374 | 382 | 379 |
Post-assembly | Chilean | Wisfal | Combined |
Total base count (bp) | 18,581,045 | 20,813,330 | 29,353,134 |
TC count | 14,815 | 17,178 | 24,144 |
Singleton count | 22,918 | 22,990 | 30,072 |
100-500 bp | 34,794 | 25,574 | 26,941 |
501-1000 bp | 14,445 | 9,940 | 10,385 |
1001-1500 bp | 3,174 | 1,573 | 1,976 |
1501-2000 bp | 1,155 | 454 | 604 |
> 2000 bp | 648 | 192 | 262 |
Minimum length (bp) | 100 | 100 | 100 |
Maximum length (bp) | 4,974 | 5,124 | 6662 |
Average length (bp) | 492 | 518 | 541 |