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Table 1 Candidate genes for which CHORI-231 was screened and subsequently 454 sequenced

From: Construction of an American mink Bacterial Artificial Chromosome (BAC) library and sequencing candidate genes important for the fur industry

Candidate gene probe Probe sequence source No. of positive signals as evaluated by no. of T7 BACs hits Phenotype(s)/condition potentially involving the candidate genes Acc. no. Coverage of the gene 454 sequenced Content of the clone(s) being sequenced SSRs in the clone(s) contig No. of contigs per gene No. of overlaping clones included for 454 sequencing Size of genomic information generated (in Kb)
KIT ligand ( KITL ) Dog and human 4 Roan, Spotted, and White phenotypes (excluding Albino but including Hedlund white, associated with deafness) JF288175 Complete KITL 13 83 2 226
Microphthalmia-associated transcription factor ( MITF ) Dog 12   JF288172 Complete MITF 23 46 2 205
KIT ( CD117 or C-KIT ) Mink & dog 4   JF288168 Clone missing exon 1 KIT 26   2 160
Melanophilin ( MLPH ) Dog 11 Silver JF288169 Complete +COL4A1 18 38 2 267
Lysosomal trafficking regulator ( LYST) Dog 4 Alutian color (associated with Chediak-Higashi syndrome) JF288176 Complete LYST 11 42 2 274
Silver ( SILV or PMEL ) Dog 1 Blue/silver phenotypes JF288177 Complete Gene rich 27 1 2 183
Tyrosinase ( TYR ) Mink 12 Albino and Himalayan type JF288171 Complete + GRM5, NOX4 &RPL23A 38 32 2 320
Protein atonal homolog 1 ( Atoh-1 ) Dog 10 Hedlund white, associated with deafness JF288173 Complete Atoh-1 23 30 3 195
Melanocortin 2/3 Receptor ( MC2R&MC3R ) Dog 6 Involved in a wide range of physiological functions, including pigmentation JF288181 Complete + MLC5R, KPNA2 &RNMT 5 13 3 97
Fibroblast growth factor 5(FGF5 ) Dog 5 Hair length JF288167 Complete + LDHR &PRDM8 22 33 2 177
R-spondin-2(RSPO2 ) Dog 7 Hair growth and coarseness JF288170 Clone missing exon 5 RSPO-2 30 24 1 153
Melanocyte stimulating hormone ( POMC ) Dog & human 7 Various types of pigmentation JF288182 Missing 15 nt in exon 2 at 5' + EFR3 & DNMT3A 17 79 2 223
Melanocortin 1 Receptor ( MC1R ) Mink & dog 1 Palomino, Pastel, Pearl & Reddish phenotypes JF288183 Complete Gene rich 2 96 1 124
Solute carrier family 24, member ( SLC24A5 ) Dog 5 Differences in skin pigmentation JF310744 Missing 9 nt in exon 1 at 5' CTXN2 &MYEX2 17 37 2 218
Agouti related protein ( AGRP ) Dog 5 Agouti and Pearl phenotypes loci were found to be closely linked JF288166 Missing 106 nt in exon 2 at 5' Gene rich 10 20 1 193
Integrin-B ( ITGB1 ) Dog 6 Aleutian Disease Virus (ADV) and Influenza susceptibility/resistance JF288179 Missing over 500 nt in several exons ITGB1 15 70 1 105
Major Histocompatibility Complex, class II, DR beta 1 ( HLA-DRB1) Dog 3   JF288174 Missing 160nt in one exon RPL7A 19 33 1 139
B-defensine ( DEFB1 ) Dog 4   JF288178 Inconsistent cds   5 51 2 186
Keratin71 ( KRT71 ) Dog 15 Coarseness JF288180 9 exons covered 10 members of the KRT family 3 101 2 56
Transmembrane inner ear ( TMIE ) Dog None Hedlund white associated with deafness - - - - - - -
Tyrosinase-related protein 1 ( TYRP1 ) Dog None Associated with pigmentation genetics - - - - - - -