Figure 4From: An RNA interference screen for identifying downstream effectors of the p53 and pRB tumour suppressor pathways involved in senescenceSilencing targets identified from the primary screen. CL3EcoR cells were infected in triplicate with lentiviruses prepared from the indicated GIPZ shRNAmir constructs targetting TMEM9B (a), LTBP2/3 (b), ATXN10 (c) and LAYN (d). Cells resistant to 6 μg/ml puromycin were isolated, reseeded and assayed for bypass of senescence by culturing at 38°C for 3 weeks. Lentiviruses prepared from the GIPZ shRNAmir Lamin A/C (v2LHS_62719) construct were used as the negative control. For TMEM9B, the mix of constructs comprised v2LHS_247318, 58957, 58958 and 58959.Back to article page