Sequence analysis of ERα, ERβ and ERα+ERβ binding sites. (A) Venn diagram showing a summary of ERα and ERβ binding sites identified in TAP-ERβ cells by ChIP-Seq. (B) Classification of ERα and β binding sites based on the presence of a perfectly or imperfectly palindromic Estrogen Response Element (ERE, green), an ERE hemipalindrome (hERE, blue) or no ERE (none, red). (C) ERE motif matrices identified in each of the three ER binding regions indicated (left), classification of the binding sites belonging to each region according to the presence of ERE (center) and grid summarizing the results of TFBS matrix enrichment (overrepresentation) analyses performed on the binding sites groups indicated (right). Z-Score cut-off was 3.0 and only TFBSs showing an over-representation score ≥4.0 in at least one of the ERE- (none) binding site groups. Light grey cells indicate a Z-Score <3.0 while dark grey cells indicate absence of the matrix.