Table 1 Summary of A. hypochondriacus 454 sequencing data trimming, assembly and annotation
Run Metrics | Total raw reads | 2,913,966 (100%) |
| Â | Total bases | 909,631,600 |
| Â | Reads after quality control and trimming | 2,700,168 (92.6%) |
| Â | Bases entering assembly | 877,153,000 (96.4%) |
Assembly | Aligned reads | 2,417,008 (89.5%) |
| Â | Aligned bases | 803,229,499 (88.3%) |
| Â | Assembled reads | 1,886,081 |
| Â | Fully assembled | 1,422,449 |
| Â | Partially assembled | 463,632 |
| Â | Singletons (5.9) | 178,636 |
| Â | Repeats | 68,980 |
| Â | Outliers | 56,216 |
| Â | Too short | 46,623 |
Isogroup Metrics | Total isogroups | 15,667 |
| Â | Average contig content | 3.0 |
| Â | Largest contig content | 22,172 |
| Â | Number with one contig | 12,739 |
| Â | Average isotig content | 1.3 |
| Â | Largest isotig content | 52 |
| Â | Number with one isotig | 12,950 |
Isotig metrics | Total isotigs | 20,408 |
| Â | Average contig content | 1.7 |
| Â | Largest contig content | 17 |
| Â | Number with one contig | 12,985 |
| Â | Number of bases | 20,710, 069 |
| Â | Average isotig size | 1, 014 |
| Â | N50 isotig size | 1,196 |
| Â | Largest isotig size | 4,762 |
Large contig metrics | Number of contigs | 15,608 |
| Â | Number of bases | 15,170,717 |
| Â | Average contig size | 971 |
| Â | N50 contig size | 1,063 |
| Â | Largest contig size | 3,379 |
All contig metrics | Number of contigs | 25,998 |
| Â | Number of bases | 18,043,010 |
Annotation (contigs/isotigs) | nr (NCBI) | 17,282 |
| Â | TAIR | 16,597 |
Annotation (singletons) | UniRef 100 | 17,440 |
| Â | UniRef 50 | 4,396 |
| Â | Amaranthaceae ESTs | 10,846 |
|  | TAIR | ≈1,000 |