Figure 4

Confirmation of hepatic TCDD-induced AhR enrichment identified by ChIP-chip analysis (FDR < 0.01) at 2 hrs by ChIP-PCR. Selected regions were chosen for verification based on position relative to a TSS, ChIP-chip fold enrichment and the presence or lack of a DRE core within the region of enrichment (A). Immunoprecipitated DNA was measured by QRTPCR and AhR enrichment was calculated as fold induction above IgG controls. The color intensity of each box represents the mean value of three independent replicates. NS = not significant compared to IgG controls (p < 0.05). 2 hr ChIP-chip enrichment values are provided in Additional File 1.