Figure 4

Physical map of the switchgrass genome region containing OsBRI1 orthologs. Contig assembly of the 6 OsBRI1 ortholog-containing BACs using HICF fingerprinting with a cutoff of e^-30 and a tolerance of 4 resulted in an assembly with 2 contigs: Contig 1 with 4 BACs, Contig 2 with 2 BACs. The two contigs represent two homoeologous genomes in switchgrass. The locations of the 12 predicted PCR products (p1 to p12) using the 12 pairs of primers (Additional file 1) designed based on sequence data from the two BAC clones, 48E09 (D10) and 65J23 (E2) are indicated on the map.