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Table 3 Differentially expressed B. cenocepacia genes selected from all conditions tested

From: Spontaneous and evolutionary changes in the antibiotic resistance of Burkholderia cenocepacia observed by global gene expression analysis

BExperimental condition from which the gene was selected Fold change observed under each microarray condition
Gene name Annotation     
Exposure to amikacin J2315 wt + 50 mg/L AMK J2315-A J2315-A + 50 mg/L AMK J2315-A + 250 mg/L AMK
BCAL1233 Putative heat shock Hsp20-related protein - 1.95 3.47 4.92
BCAL1234 Putative heat shock protein - - 3.00 4.43
BCAL1755 Conserved hypothetical protein 2.44 - 4.74 3.47
BCAL1756 Putative metal dependent phosphohydrolase 1.78 - 2.95 3.14
BCAL1919 ClpB heat-shock protein - - - 3.20
BCAL2442 Chaperone protein HtpG - - - 2.39
BCAL3146 60 kDa chaperonin 1 - - - 2.47
BCAL3147 10 kDa chaperonin 1 - - - 2.96
BCAL3148 Polyketide cyclase/dehydrase and lipid transport Family - 2.14 3.27 1.95
BCAL3149 Outer membrane lipoprotein carrier protein LolA - 2.86 4.22 2.31
BCAL3150 Putative exported protein - 3.15 4.34 -
BCAL3151 putative transmembrane anti-sigma factor - 2.76 4.43 2.31
BCAL3152 putative RNA polymerase sigma factor, ECF subfamily - 2.94 4.77 3.40
BCAL3153 Putative lipoprotein - 3.47 5.82 4.45
BCAL3270 Putative DnaK chaperone protein - - - 2.76
BCAM0829a RimL, Acetyltransferases, including N-acetylases of ribosomal proteins - 2.51 2.46 2.14
BCAM0831 ABC transporter ATP-binding membrane protein - 1.91 2.43 3.01
BCAS0637 60 kDa chaperonin 3 - - - 6.62
BCAS0638 10 kDa chaperonin 3 - - - 7.88
Exposure to meropenem J2315 wt + 2 mg/L MER J2315-M J2315-M + 2 mg/L MER J2315-M + 8 mg/L MER
BCAL0212 Putative phenylacetic acid degra-dation NADH oxidoreductase PaaE - - - 3.32
BCAL0213 Phenylacetic acid degradation protein PaaD - - - 6.61
BCAL0214 Phenylacetic acid degradation protein PaaC - - - 3.14
BCAL0215 Phenylacetic acid degradation protein PaaB - 8.01 6.48 3.81
BCAL0216 Phenylacetic acid degradation protein PaaA - 14.83 10.03 6.79
BCAL0404 Phenylacetate-coenzyme A ligase - - - 2.58
BCAL0405 Phenylacetic acid degradation protein PaaI - - - 2.75
BCAL0406 Probable enoyl-CoA hydratase PaaG - 6.28 - 3.63
BCAL0407 Beta-ketoadipyl CoA thiolase - - - 4.48
BCAL0408 Putative phenylacetic acid degradation oxidoreductase paaZ - 9.93 7.93 5.75
BCAL0409 Putative phenylacetic acid degra-dation enoyl-CoA hydratase PaaF - 2.67 - -
BCAL1804 Major Facilitator Superfamily protein - -3.45 -2.70 -1.85
BCAL1805 Putative sugar kinase - -4.76 -4.35 -2.22
BCAL1806 Conserved hypothetical protein - -14.29 -9.90 -5.26
BCAM1356 Putative gluconate 2-dehydrogenase subunit 3 - -25.64 -29.50 -12.85
BCAM1357 Gluconate 2-dehydrogenase flavoprotein subunit - -7.14 -7.30 -5.52
BCAM1358 Gluconate 2-dehydrogenase cytochrome c subunit - -24.69 -25.38 -9.80
BCAM1710 Putative enoyl-CoA hydratase/isomerase - 3.23 - 4.32
BCAM1711 Phenylacetate-coenzyme A ligase paaK - 12.48 11.01 5.24
BCAM2165 Putative beta-lactamase, class A 53.35 - 60.30 60.62
BCAS0128 ABC transporter ATP-binding protein -2.27 -2.04 - -3.70
BCAS0129 Putative binding-protein-dependent transport system component -2.56 -2.13 - -4.98
BCAS0130 Putative ABC transporter substrate-binding protein -2.94 - -2.48 -6.25
BCAS0156 Beta-lactamase, class C 116.10 - 133.10 61.12
Exposure to trimethoprim sulfamethoxazole J2315 wt + 10/50 mg/L TMP/SMX J2315-T J2315-T + 10/50 mg/L TMP/SMX J2315-T + 60/300 mg/L TMP/SMX
BCAL0145 Adenosylhomocysteinase 1.77 - - 2.26
BCAL0147 5,10-methylenetetrahydro-folate reductase 1.61 - - 2.07
BCAM0186 Lectin 2.90 - - -
BCAM1869 Conserved hypothetical protein - -3.68 -4.13 -2.09
BCAM1870 N-acylhomoserine lactone synthase CepI - -15.48 -14.95 -3.51
BCAM1871 Conserved hypothetical protein 2.03 -14.88 -12.90 -3.73
BCAM2549 Multidrug efflux system outer membrane protein - 138.20 111.80 35.80
BCAM2550 Multidrug efflux system transporter protein - 108.80 103.60 30.79
BCAM2551 Multidrug efflux system transport protein CeoA - 296.90 341.50 55.38
BCAM2552 Putative hydrolase - 187.40 167.50 45.93
BCAM2554 LysR family regulatory protein - 4.34 4.36 2.40
BCAS0292 Conserved hypothetical protein 3.41 -6.02 -4.03 -
BCAS0293 Nematocidal protein AidA 8.56 -6.02 -5.65 -2.67
BCAS0409 ZmpA 1.99 - - -
Exposure to chlorpromazine J2315 wt + 0.05 mM CPZ J2315 wt + 0.05 mM CPZ + 50 mg/L AMK   
BCAL1233 Putative heat shock Hsp20-related protein - 2.98   
BCAL1234 Putative heat shock protein - 2.40   
BCAL1755 Conserved hypothetical protein - 2.07   
BCAL1756 Conserved hypothetical protein 2.04 1.82   
BCAM0923 Putative lipoprotein 11.00 11.33   
BCAM0924 Two-component regulatory system, response regulator protein 4.85 4.63   
BCAM0925 Multidrug efflux system outer membrane protein 12.80 16.51   
BCAM0926 Multidrug efflux system transporter protein 17.75 27.45   
BCAM0927 Multidrug efflux system transport protein 13.75 22.43   
BCAM2186 Putative macrolide-specific efflux system transport protein 1.96 2.04   
BCAM2187 Putative ThiJ/PfpI family protein 1.60 2.02   
BCAS0167 Squalene-hopene cyclase 4.36 5.35   
BCAS0168 TetR family regulatory protein 2.33 2.81   
BCAS0638 10 kDa chaperonin 3   4.18