Comparative high-throughput transcriptome sequencing and development of SiESTa, the Silene EST annotation database

Table 6 Library SNP content

Library	SlM	SlF	SlFf	SdM	SdF	SmM	SvH	Ds
Contigs*	2909	5486	2287	2993	2912	4982	5028	6094
Contigs with SNPs	1221	1517	976	1333	1078	1709	1619	2513
SNPs	6648	6308	5576	6307	4653	7361	7381	12282
Substitutions	4847	5165	3873	5356	3516	5681	6402	9927
% Transitions/transversions	52/48	61/39	47/53	63/37	56/44	57/43	60/40	61/39
% heterozygous positions	0.39	0.19	0.43	0.38	0.28	0.26	0.27	0.32

* Only contigs assembled from at least 4 reads were considered. The total length of these contigs was used to calculate the percentage of heterozygous positions. All SNPs that are not due to substitutions are indels.

ISSN: 1471-2164