Figure 3

Generation and characterization of matched gene groups displaying differential KRAB/KAP1 silencing phenotypes. (A) Matched gene groups with differential KRAB/KAP1-silencing phenotypes. The cumulative histograms illustrate the distribution of repressible (blue) and non-repressible (red) LV-TrapSil and MLV-TrapSil integrants in the transcribed region of genes trapped multiple times. Three groups were distinguished based on the pattern of repressible and non-repressible integrants over 20 kb. Group 1 genes harbored mainly repressing integrants ("long-range repression"), while group 3 contained mostly non-repressible integrants ("absence of repression"). Group 2 genes exhibited an intermediate phenotype, with repressing integrants clustered over the first 10 kb of their transcribed region ("limited repression"). (B) The three gene groups (group 1: "long-range repression", group 2: "limited repression" and group 3: "absence of repression") were analyzed by ROC curves as described in Figure 2 for genomic features over various DNA stretches. (C) Comparison of mean expression levels of the three gene groups based on publicly available microarray data. Group 1: "long-range repression", group 2: "limited repression" and group 3: "absence of repression". Statistical comparisons were made with a non-parametric Wilcoxon test. P-Value Legend: ** p < 0.01.