Table 2 454 Read Pre-processing

Titanium 454 Plates

0.5

0.5

Raw Reads (unbroken)

654,164

568,399

Raw Sequence (Mbp)

239.5

205.3

Average Raw Read Length (bp)

366.0

361.0

¹Raw Coverage

79.8

68.4

Stage I - Split Pairs

Reads

910,019

568,399

Sequence (Mbp)

201

205.3

Average Read Length (bp)

220.9

361.0

Stage I Coverage

67.0

68.4

Stage II - Trim/Contamination Removal

Reads

496,756

338,957

Sequence (Mbp)

121.7

125.4

Average Read Length (bp)

244.9

367.9

Stage II Coverage

40.6

41.8

Stage III - Mate Pair Detection

Reads

371,430

338,957

Sequence (Mbp)

67.3

125.4

Average Read Length (bp)

181.3

367.9

Stage III Coverage

22.4

41.8