Conserved function tendencies within the gene-associations of Sigma-54, its EBP54s and the Sigma-54 promoter. The highly represented and cross-phylum conserved metabolic reactions were mapped using iPATH . The reactions that relate to only Firmicutes are colored green, those that relate to diderm organisms only are colored yellow and those reactions represented in both monoderm and diderm species are given in orange. The routes associated with phospholipid, peptidoglycan and lipopolysaccharide biosynthesis are indicated and the related precursors are given in blue boxes. The metabolites that are associated to the recovered reactions fall in 3 distinct categories. i) CoA-related: A1, acetyl-CoA; A2, propanoyl-CoA; A3, propenoyl-CoA; A4, 3-hydroxypropanoyl-CoA; A5, 2-methylpropanoyl-CoA; A6, 3-methylbutanoyl-CoA; A7, 2-methylbutanoyl-CoA; A8, (R)-2-methyl-3-oxopropanoyl-CoA; A9, 2-butenoyl-CoA; A10, (S)-3-hydroxybutanoyl-CoA; A11, succinyl-CoA; A12, glutaryl-CoA; A13, 3alpha,7alpha-dihydroxy-5beta-cholestanoyl-CoA; A14, 3-oxoadipyl-CoA; A15, hexadecanoyl-CoA; A16a, acetoacetyl-CoA; A16b, acetoacetyl-[acp]; A17, butanoyl-CoA. ii) carboxylates: C1, acetate; C2, 3-oxopropanoate; C3 glycolate; C4, malate; C5, 3-methyl-2-oxobutanoate; C6, 4-methyl-2-oxopentanoate; C7, 3-methyl-2-oxopentanoate; C8, (S)-methylmalonate semialdehyde; C9, L-aspartate; C10, butanoate; C11, 4-aminobutanoate; C12, L-glutamate; C13, succinate semialdehyde; C14, succinate; C15, hexadecanoate; C16, isocitrate; C17, citrate; C18, oxaloacetate; C19, 3-phospho-D-glycerate; C20, acetoacetate; C21, salicylate; C22, 3-oxoadipate; C23, 3,4-dihydroxymandelaldehyde; C24, chorismate; C25, 6-oxohexanoate; C26, 2-oxoglutarate. iii) amino-group containing: N1, histamine; N2, anthranilate; N3, 5-hydroxytryptamine; N4, 2-amino-4-hydroxy-6-(erythro-1,2,3-trihydroxypropyl)dihydropteridinetriphosphate; N5, Nicotinate; N6, 1,4-butanediamine; N7, 2-hydroxyethyl-ThPP.