CRL1 expression is induced by auxin within 1 hour. Time course of transcription of CRL1 and IAA4 genes in response to mock or IAA treatment (0, 1, 3 or 6 hours) in WT and crl1 mutant assayed by RT-qPCR. Y-axis indicates the relative expression level at each timepoint relative to timepoint "Ø" (before treatment). IAA4: early-auxin responsive gene, control of IAA treatment. h, hour after treatment. Values represent the mean obtained from 3 independent auxin treatment experiments, bars represent the standard deviation. Mean values were compared between WT and crl1 mutant for each time point. A significant difference was noted with one (p-value < 0.1) or two plus signs (p-value < 0.05).