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Table 3 Evaluation of SSR primer pairs in four carrot genetic stocks used to develop the EST library

From: De novo assembly and characterization of the carrot transcriptome reveals novel genes, new markers, and genetic diversity

PCR results

Number of primers

Percentage of all primer tested

Percentage of amplified primer

SSR

   

Tested

114

  

Amplified

102

89.5

 

Single product

99

86.8

97

   Single expected product

75

65.8

74

   Single larger product

24

21.1

24

Multiple product

3

2.6

3

Polymorphic*

26

  
  1. *out of 31 tested.