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Figure 8 | BMC Genomics

Figure 8

From: Genome-wide survey of yeast mutations leading to activation of the yeast cell integrity MAPK pathway: Novel insights into diverse MAPK outcomes

Figure 8

Transcriptional response observed in mutants pmt2 Δ and ssd1 Δ is dependent on Slt2 activity. (a) Expression of MLP1-lacZ, CWP1-lacZ and SED1-lacZ was determined in wild-type strain and pmt2 Δ, pmt2 Δrlm1 Δ, pmt2 Δslt2 Δ, ssd1 Δ, ssd1 Δrlm1 Δ and ssd1 Δslt2 Δ mutant cells growing in a rich medium under standard conditions. (b) The MLP1-lacZ reporter plasmid was co-transformed with centromeric plasmids bearing the wild-type SLT2 allele, inactive mutant alleles of SLT2 (slt2/mpk1-K54R and slt2/mpk1-TA/YF) or the empty vector (pRS315) into pmt2 Δslt2 Δ and ssd1 Δslt2 Δ strains, and β-galactosidase activity was measured in crude extracts. Each value represents the mean and standard deviation of three independent transformants.

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