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Table 4 An overall validation of microarray data by qRT-PCR

From: Transcription analysis on response of swine lung to H1N1 swine influenza virus

Gene GenBank ID Microarray FC qRT-PCR FC
TAP1 NM_001044581 2.46 2.83
SAA2 CB475095 8.42 2.86
C4 CF366280 2.95 3.53
CD2 NM_213776.1 2.93 6.43
IRG6 NM_213817.1 3.84 32.50
TCN1 BX675338 7.37 3.56
ISG20 NM_001005351.1 5.41 2.15
PR39 NM_214450 13.51 16.48
PMAP23 EW419136 21.7 13.07
NPG4 NM_213863 8.24 21.99
TGFB2 NM_214015.1 -2.55 -3.83
VEGFA EU714324 -2.42 -6.20
TLR5 NM_001123202 -2.10 -5.78
  1. Fold changes of gene expression in the porcine lung 3 days following H1N1 SwIV infection compared with the controls. The fold changes were calculated by the formula of 2-delta-delta Ct, data was means ± SD of triplicate reactions for each gene transcript. The expression level of GAPDH was assayed for normalization during quantitative PCR.