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Table 5 A validation of Toll-like receptors and IFN-stimulated genes by qRT-PCR

From: Transcription analysis on response of swine lung to H1N1 swine influenza virus

Gene

GenBank ID

Microarray FC

qRT-PCR FC

TLR2

NM_213761.1

2.87

4.61

TLR3

NM_001097444.1

7.33

3.42

TLR8

NM_214187

2.68

2.44

TLR9

NM_213958

2.20

1.64

IRF3

NM_213770.1

4.32

6.33

IRF7

NM_001097428

3.25

11.1

Mx1

NM_214061

3.24

10.36

ISG15

EU584557.1

3.45

5.21

ISG20

NM_001005351.1

5.41

2.15

ISG54

XM_001928671.2

2.73

6.27

ISG56

XM_001928724;

3.39

3.43

ISG60

XM_001928703

2.47

5.70

GBP1

NM_001128473

2.78

12.0

OASL

NM_001031790

5.80

2.05

OAS2

NM_001031796

2.05

28.80

  1. qRT-PCR results of 4 Toll-like receptors and 11 IFN-stimulated genes in the SwIV -infected pigs compared with the controls. Gene expression is depicted as relative expression compared to GAPDH gene expression.