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Table 4 Concentrations and vehicles used for each reference chemical

From: A genomic biomarker signature can predict skin sensitizers using a cell-based in vitro alternative to animal tests

Compound

Abbreviation

Vehicle

Max solubility

(μM)

Rv90

(μM)

Concentration

in culture (μM)

Sensitizers

     

2,4-Dinitrochlorobenzene

DNCB

DMSO

-

4

4

Oxazolone

OXA

DMSO

250

-

250

Potassium dichromate

PD

Water

51.02

1.5

1.5

Kathon CG (MC/MCI)1

KCG

Water

-

0.0035%

0.0035%

Formaldehyde

FA

Water

-

80

80

2-Aminophenol

2AP

DMSO

-

100

100

2-nitro-1,4-Phenylendiamine

NPDA

DMSO

-

300

300

p-Phenylendiamine

PPD

DMSO

566

75

75

Hexylcinnamic aldehyde

HCA

DMSO

32.34

-

32.24

2-Hydroxyethyl acrylate

2HA

Water

-

100

100

2-Mercaptobenzothiazole

MBT

DMSO

250

-

250

Glyoxal

GO

Water

-

300

300

Cinnamaldehyde

CALD

Water

-

120

120

Isoeugenol

IEU

DMSO

641

300

300

Ethylendiamine

EDA

Water

-

-

500

Resorcinol

RC

Water

-

-

500

Cinnamic alcohol

CALC

DMSO

500

-

500

Eugenol

EU

DMSO

649

300

300

Penicillin G

PEN G

Water

-

-

500

Geraniol

GER

DMSO

-

-

500

Non-sensitizers

     

1-Butanol

BUT

DMSO

-

-

500

4-Aminobenzoic acid

PABA

DMSO

-

-

500

Benzaldehyde

BA

DMSO

250

-

250

Chlorobenzene

CB

DMSO

98

-

98

Diethyl phthalate

DP

DMSO

50

-

50

Dimethyl formamide

DF

Water

-

-

500

Ethyl vanillin

EV

DMSO

-

-

500

Glycerol

GLY

Water

-

-

500

Isopropanol

IP

Water

-

-

500

Lactic acid

LA

Water

-

-

500

Methyl salicylate

MS

DMSO

-

-

500

Octanoic acid

OA

DMSO

504

-

500

Propylene glycol

PG

Water

-

-

500

Phenol

PHE

Water

-

-

500

p-Hydroxybenzoic acid

HBA

DMSO

250

-

250

Potassium permanganate

PP

Water

38

-

38

Salicylic acid

SA

DMSO

-

-

500

Sodium dodecyl sulphate

SDS

Water

-

200

200

Tween 80

T80

DMSO

-

-

500

Zinc sulphate

ZS

Water

126

-

126

  1. List of concentrations and vehicles used for each testing compound. 1) Kathon CG is a mixture of the compounds MC and MCI. The concentration of this mixture is given in %.