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Table 4 Concentrations and vehicles used for each reference chemical

From: A genomic biomarker signature can predict skin sensitizers using a cell-based in vitro alternative to animal tests

Compound Abbreviation Vehicle Max solubility (μM) Rv90 (μM) Concentration in culture (μM)
Sensitizers      
2,4-Dinitrochlorobenzene DNCB DMSO - 4 4
Oxazolone OXA DMSO 250 - 250
Potassium dichromate PD Water 51.02 1.5 1.5
Kathon CG (MC/MCI)1 KCG Water - 0.0035% 0.0035%
Formaldehyde FA Water - 80 80
2-Aminophenol 2AP DMSO - 100 100
2-nitro-1,4-Phenylendiamine NPDA DMSO - 300 300
p-Phenylendiamine PPD DMSO 566 75 75
Hexylcinnamic aldehyde HCA DMSO 32.34 - 32.24
2-Hydroxyethyl acrylate 2HA Water - 100 100
2-Mercaptobenzothiazole MBT DMSO 250 - 250
Glyoxal GO Water - 300 300
Cinnamaldehyde CALD Water - 120 120
Isoeugenol IEU DMSO 641 300 300
Ethylendiamine EDA Water - - 500
Resorcinol RC Water - - 500
Cinnamic alcohol CALC DMSO 500 - 500
Eugenol EU DMSO 649 300 300
Penicillin G PEN G Water - - 500
Geraniol GER DMSO - - 500
Non-sensitizers      
1-Butanol BUT DMSO - - 500
4-Aminobenzoic acid PABA DMSO - - 500
Benzaldehyde BA DMSO 250 - 250
Chlorobenzene CB DMSO 98 - 98
Diethyl phthalate DP DMSO 50 - 50
Dimethyl formamide DF Water - - 500
Ethyl vanillin EV DMSO - - 500
Glycerol GLY Water - - 500
Isopropanol IP Water - - 500
Lactic acid LA Water - - 500
Methyl salicylate MS DMSO - - 500
Octanoic acid OA DMSO 504 - 500
Propylene glycol PG Water - - 500
Phenol PHE Water - - 500
p-Hydroxybenzoic acid HBA DMSO 250 - 250
Potassium permanganate PP Water 38 - 38
Salicylic acid SA DMSO - - 500
Sodium dodecyl sulphate SDS Water - 200 200
Tween 80 T80 DMSO - - 500
Zinc sulphate ZS Water 126 - 126
  1. List of concentrations and vehicles used for each testing compound. 1) Kathon CG is a mixture of the compounds MC and MCI. The concentration of this mixture is given in %.