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Figure 1 | BMC Genomics

Figure 1

From: Combining blue native polyacrylamide gel electrophoresis with liquid chromatography tandem mass spectrometry as an effective strategy for analyzing potential membrane protein complexes of Mycobacterium bovis bacillus Calmette-Guérin

Figure 1

BN PAGE of membrane fractions from M. bovis BCG. Membrane fractions were incubated with detergents for at least one hour at 4°C. Lane 1 was High Molecular Weight (HMW) calibration and lane 2, 3 were treated with Triton X-100 (0.2% and 0.1% (w/v), respectively). Lane 4, 5 were solubilized with DDM (2% and 1% (w/v), respectively). Gel gradient was 4-16%. The gel was run according to the protocol given in Methods.

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BMC Genomics

ISSN: 1471-2164

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