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Table 1 List of M. bovis BCG potential multiprotein complexes and proteins identified in this study.

From: Combining blue native polyacrylamide gel electrophoresis with liquid chromatography tandem mass spectrometry as an effective strategy for analyzing potential membrane protein complexes of Mycobacterium bovis bacillus Calmette-Guérin

proteina)

description

peptideb)

SCc)

pId)

Mr(kDa)e)

scoref)

TMHg)

Gravyh)

functioni)

bandj)

spotk)

Pks2

polyketide synthase

10

4.09

5.18

227.13

60.02

0

-0.001

1

I

1

MmpL8

integral membrane transport protein

3

4.78

9.85

116.28

35.23

12

0.330

3

I

2

FadD23

fatty-acid-CoA ligase

4

8.56

5.42

62.84

29.57

2

0.064

1

I

3

PapA1

polyketide synthase associated protein

4

11.35

5.33

56.13

27.47

0

0.013

1

I

4

BCG_3890c

putative transposase

2

3.19

11.94

46.11

31.02

0

-0.577

5

I

5

LpqW

putative lipoprotein

3

4.25

5.23

66.37

104.53

0

0.027

3

II

6

AtpA

ATP synthase alpha chain

14

22.60

4.89

59.48

1883.30

0

-0.207

7

II

7

AtpD

ATP synthase beta chain

19

46.50

4.71

53.18

2917.93

0

-0.168

7

II

8

AtpH

ATP synthase delta chain

9

18.40

5.28

48.83

285.95

1

0.027

7

II

9

LipO

putative esterase

4

36.07

10.59

46.10

36.07

0

-0.113

7

II

10

AtpG

ATP synthase gamma chain

9

21.30

5.26

33.93

593.00

0

-0.267

7

II

12

AtpB

ATP synthase a chain

2

12.80

6.14

27.51

80.91

5

0.818

7

II,IV

13,25

AtpF

ATP synthase b chain

6

39.80

5.11

18.32

115.66

1

0.040

7

II

15

AtpC

ATP synthase epsilon chain

5

47.10

4.55

13.13

43.53

0

-0.061

7

II

16

AtpE

ATP synthase c chain

2

49.40

4.78

8.06

24.67

2

1.016

7

II

17

PpsC

phenolpthiocerol synthesis type-I polyketide synthase

8

5.30

4.92

231.22

59.56

0

0.028

1

III

18

PpsA

phenolpthiocerol synthesis type-I polyketide synthase

12

7.25

5.02

199.69

132.56

0

-0.045

1

III

19

EmbB

integral membrane indolylacetylinositol arabinosyltransferase

4

2.55

10.14

118.14

40.51

12

0.312

3

IV

21

EmbC

integral membrane indolylacetylinositol arabinosyltransferase

7

6.31

10.42

117.83

59.83

12

0.246

3

IV

22

EmbA

integral membrane indolylacetylinositol arabinosyltransferase

3

3.56

10.12

115.92

39.95

12

0.338

3

IV

23

BCG_2759c

putative membrane alanine rich protein

4

12.22

12.05

29.06

98.48

2

0.113

10

IV

24

Pks7

polyketide synthase

12

8.09

5.29

221.75

154.53

0

0.213

1

V

26

Pks8

polyketide synthase

6

4.50

5.21

167.47

41.55

0

0.264

1

V

27

DnaK

chaperone protein

11

26.72

4.70

66.79

202.48

0

-0.368

0

VI

28

GroEL2

60 KDA chaperonin 2

5

11.30

4.70

56.69

82.72

0

-0.091

0

VI

29

GroEL1

60 kDa chaperonin 1

6

9.46

4.84

55.84

61.98

0

0.110

0

VI

30

DnaJ2

chaperone protein

3

10.21

6.24

41.03

31.23

0

-0.237

0

VI,II

11,31

FurA

ferric uptake regulation protein

2

15.33

5.46

16.53

23.78

0

0.013

9

VI

32

MmpL11

transmembrane transport protein

3

2.48

10.14

104.06

34.28

12

0.337

3

VII

33

BCG_0241c

transmembrane protein

3

6.32

10.29

45.89

20.18

8

0.323

3

VII

34

BCG_0238c

hypothetical protein

2

8.98

7.75

18.52

44.45

0

-0.292

10

VII

35

RplB

50S ribosomal protein L2

9

29.30

11.94

30.62

100.45

0

-0.851

2

VIII

36

RpsD

30S ribosomal protein S4

2

9.50

10.33

23.46

95.47

0

-0.722

2

VIII

37

RpsE

30S ribosomal protein S5

3

13.20

10.74

22.93

24.67

0

-0.171

2

VIII

38

RplE

50S ribosomal protein L5

5

19.30

10.52

21.01

73.79

0

-0.344

2

VIII

39

RplV

50S ribosomal protein L22

8

44.20

12.24

20.37

162.57

0

-0.494

2

VIII

40

RplM

50S ribosomal protein L13

2

17.00

10.41

16.34

22.52

0

-0.418

2

VIII

41

RplR

50S ribosomal protein L18

6

46.70

12.10

13.18

133.98

0

-0.329

2

VIII

42

SdhA

succinate dehydrogenase (flavoprotein subunit)

20

36.78

5.65

65.24

1062.30

0

-0.246

7

IX

43

SdhB

succinate dehydrogenase (iron-sulphur protein subunit)

4

12.55

8.93

30.01

211.54

0

-0.154

7

IX

44

  1. a) Protein name in M. bovis BCG database.
  2. b) Number of identified unique peptides obtained for a particular protein.
  3. c) Sequence coverage in percent of the identified protein.
  4. d) Isoelectric point of the protein that calculated from its amino acid sequence.
  5. e) Molecular weight in kDa of the respective protein.
  6. f) The Mascot score of the protein identification.
  7. g) Transmembrane helices predicted by TMHMM 2.0 program.
  8. h) Grand average of hydrophobicity predicted by ProtParam.
  9. i) Functional classification based on the Pasteur Institute functional classification tree.
  10. j) Number of BN gel bands for complex identification.
  11. k) Number of spots for protein identification.
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BMC Genomics

ISSN: 1471-2164

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